Identification of murine CD8 T cell epitopes in codon-optimized SARS-associated coronavirus spike protein.
Identifieur interne : 004805 ( Main/Exploration ); précédent : 004804; suivant : 004806Identification of murine CD8 T cell epitopes in codon-optimized SARS-associated coronavirus spike protein.
Auteurs : Yan Zhi [États-Unis] ; Gary P. Kobinger ; Heather Jordan ; Katie Suchma ; Susan R. Weiss ; Hao Shen ; Gregory Schumer ; Guangping Gao ; Julie L. Boyer ; Ronald G. Crystal ; James M. WilsonSource :
- Virology [ 0042-6822 ] ; 2005.
Descripteurs français
- KwdFr :
- Adénovirus humains (génétique), Adénovirus humains (immunologie), Adénovirus humains (métabolisme), Animaux, Antigène d'histocompatibilité H2-D, Antigènes H-2 (métabolisme), Cartographie épitopique, Cellules Vero, Codon, Données de séquences moléculaires, Déterminants antigéniques des lymphocytes T (immunologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Immunisation, Lignée cellulaire, Lymphocytes T CD8+ (immunologie), Peptides (), Peptides (immunologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Souris, Souris de lignée BALB C, Souris de lignée C57BL, Syndrome respiratoire aigu sévère (), Séquence d'acides aminés, Vaccins antiviraux (administration et posologie), Vaccins antiviraux (immunologie), Vecteurs génétiques (administration et posologie), Vecteurs génétiques (immunologie), Virus du SRAS (immunologie).
- MESH :
- administration et posologie : Vaccins antiviraux, Vecteurs génétiques.
- génétique : Adénovirus humains, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- immunologie : Adénovirus humains, Déterminants antigéniques des lymphocytes T, Glycoprotéines membranaires, Lymphocytes T CD8+, Peptides, Protéines de l'enveloppe virale, Vaccins antiviraux, Vecteurs génétiques, Virus du SRAS.
- métabolisme : Adénovirus humains, Antigènes H-2.
- Animaux, Antigène d'histocompatibilité H2-D, Cartographie épitopique, Cellules Vero, Codon, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Humains, Immunisation, Lignée cellulaire, Peptides, Souris, Souris de lignée BALB C, Souris de lignée C57BL, Syndrome respiratoire aigu sévère, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Adenoviruses, Human (genetics), Adenoviruses, Human (immunology), Adenoviruses, Human (metabolism), Amino Acid Sequence, Animals, CD8-Positive T-Lymphocytes (immunology), Cell Line, Chlorocebus aethiops, Codon, Epitope Mapping, Epitopes, T-Lymphocyte (immunology), Genetic Vectors (administration & dosage), Genetic Vectors (immunology), H-2 Antigens (metabolism), Histocompatibility Antigen H-2D, Humans, Immunization, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Molecular Sequence Data, Peptides (chemistry), Peptides (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Vaccines (administration & dosage), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Viral Vaccines.
- chemical , chemistry : Peptides.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Epitopes, T-Lymphocyte, Membrane Glycoproteins, Peptides, Viral Envelope Proteins, Viral Vaccines.
- chemical , metabolism : H-2 Antigens.
- chemical : Codon, Histocompatibility Antigen H-2D, Spike Glycoprotein, Coronavirus.
- administration & dosage : Genetic Vectors.
- genetics : Adenoviruses, Human.
- immunology : Adenoviruses, Human, CD8-Positive T-Lymphocytes, Genetic Vectors, SARS Virus.
- metabolism : Adenoviruses, Human.
- prevention & control : Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Animals, Cell Line, Chlorocebus aethiops, Epitope Mapping, Humans, Immunization, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Molecular Sequence Data, Vero Cells.
Abstract
The causative agent of severe acute respiratory syndrome (SARS) has been identified as a new type of coronavirus, SARS-associated coronavirus (SARS-CoV). CD8 T cells play an important role in controlling diseases caused by other coronaviruses and in mediating vaccine-induced protective immunity in corresponding animal models. The spike protein, a main surface antigen of SARS-CoV, is one of the most important antigen candidates for vaccine design. Overlapping peptides were used to identify major histocompatibility complex class I-restricted epitopes in mice immunized with vectors encoding codon-optimized SARS-CoV spike protein. CD8 T-cell responses were mapped to two H-2(b)-restricted epitopes (S436-443 and S525-532) and one H-2(d)-restricted epitope (S366-374). The identification of these epitopes will facilitate the evaluation of vaccine strategies in murine models of SARS-CoV infection. Furthermore, codon and promoter optimizations can greatly enhance the overall immunogenicity of spike protein in the context of replication-defective human and simian adenoviral vaccine carriers. The optimized recombinant adenoviral vaccine vectors encoding spike can generate robust antigen-specific cellular immunity in mice and may potentially be useful for control of SARS-CoV infection.
DOI: 10.1016/j.virol.2005.01.050
PubMed: 15823604
Affiliations:
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Le document en format XML
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Boyer</name></author><author><name sortKey="Crystal, Ronald G" sort="Crystal, Ronald G" uniqKey="Crystal R" first="Ronald G" last="Crystal">Ronald G. Crystal</name></author><author><name sortKey="Wilson, James M" sort="Wilson, James M" uniqKey="Wilson J" first="James M" last="Wilson">James M. 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(immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C57BL</term><term>Molecular Sequence Data</term><term>Peptides (chemistry)</term><term>Peptides (immunology)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Vaccines (administration & dosage)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adénovirus humains (génétique)</term><term>Adénovirus humains (immunologie)</term><term>Adénovirus humains (métabolisme)</term><term>Animaux</term><term>Antigène d'histocompatibilité H2-D</term><term>Antigènes H-2 (métabolisme)</term><term>Cartographie épitopique</term><term>Cellules Vero</term><term>Codon</term><term>Données de séquences moléculaires</term><term>Déterminants antigéniques des lymphocytes T (immunologie)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Immunisation</term><term>Lignée cellulaire</term><term>Lymphocytes T CD8+ (immunologie)</term><term>Peptides ()</term><term>Peptides (immunologie)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris de lignée C57BL</term><term>Syndrome respiratoire aigu sévère ()</term><term>Séquence d'acides aminés</term><term>Vaccins antiviraux (administration et posologie)</term><term>Vaccins antiviraux (immunologie)</term><term>Vecteurs génétiques (administration et posologie)</term><term>Vecteurs génétiques (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" 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Vectors</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Vaccins antiviraux</term><term>Vecteurs génétiques</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adenoviruses, Human</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Adénovirus humains</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Adénovirus humains</term><term>Déterminants antigéniques des lymphocytes T</term><term>Glycoprotéines membranaires</term><term>Lymphocytes T CD8+</term><term>Peptides</term><term>Protéines de l'enveloppe virale</term><term>Vaccins antiviraux</term><term>Vecteurs génétiques</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Adenoviruses, Human</term><term>CD8-Positive T-Lymphocytes</term><term>Genetic Vectors</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Adenoviruses, Human</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Adénovirus humains</term><term>Antigènes H-2</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Epitope Mapping</term><term>Humans</term><term>Immunization</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C57BL</term><term>Molecular Sequence Data</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Antigène d'histocompatibilité H2-D</term><term>Cartographie épitopique</term><term>Cellules Vero</term><term>Codon</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Immunisation</term><term>Lignée cellulaire</term><term>Peptides</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris de lignée C57BL</term><term>Syndrome respiratoire aigu sévère</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The causative agent of severe acute respiratory syndrome (SARS) has been identified as a new type of coronavirus, SARS-associated coronavirus (SARS-CoV). CD8 T cells play an important role in controlling diseases caused by other coronaviruses and in mediating vaccine-induced protective immunity in corresponding animal models. The spike protein, a main surface antigen of SARS-CoV, is one of the most important antigen candidates for vaccine design. Overlapping peptides were used to identify major histocompatibility complex class I-restricted epitopes in mice immunized with vectors encoding codon-optimized SARS-CoV spike protein. CD8 T-cell responses were mapped to two H-2(b)-restricted epitopes (S436-443 and S525-532) and one H-2(d)-restricted epitope (S366-374). The identification of these epitopes will facilitate the evaluation of vaccine strategies in murine models of SARS-CoV infection. Furthermore, codon and promoter optimizations can greatly enhance the overall immunogenicity of spike protein in the context of replication-defective human and simian adenoviral vaccine carriers. The optimized recombinant adenoviral vaccine vectors encoding spike can generate robust antigen-specific cellular immunity in mice and may potentially be useful for control of SARS-CoV infection.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region></list><tree><noCountry><name sortKey="Boyer, Julie L" sort="Boyer, Julie L" uniqKey="Boyer J" first="Julie L" last="Boyer">Julie L. Boyer</name><name sortKey="Crystal, Ronald G" sort="Crystal, Ronald G" uniqKey="Crystal R" first="Ronald G" last="Crystal">Ronald G. Crystal</name><name sortKey="Gao, Guangping" sort="Gao, Guangping" uniqKey="Gao G" first="Guangping" last="Gao">Guangping Gao</name><name sortKey="Jordan, Heather" sort="Jordan, Heather" uniqKey="Jordan H" first="Heather" last="Jordan">Heather Jordan</name><name sortKey="Kobinger, Gary P" sort="Kobinger, Gary P" uniqKey="Kobinger G" first="Gary P" last="Kobinger">Gary P. Kobinger</name><name sortKey="Schumer, Gregory" sort="Schumer, Gregory" uniqKey="Schumer G" first="Gregory" last="Schumer">Gregory Schumer</name><name sortKey="Shen, Hao" sort="Shen, Hao" uniqKey="Shen H" first="Hao" last="Shen">Hao Shen</name><name sortKey="Suchma, Katie" sort="Suchma, Katie" uniqKey="Suchma K" first="Katie" last="Suchma">Katie Suchma</name><name sortKey="Weiss, Susan R" sort="Weiss, Susan R" uniqKey="Weiss S" first="Susan R" last="Weiss">Susan R. Weiss</name><name sortKey="Wilson, James M" sort="Wilson, James M" uniqKey="Wilson J" first="James M" last="Wilson">James M. Wilson</name></noCountry><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Zhi, Yan" sort="Zhi, Yan" uniqKey="Zhi Y" first="Yan" last="Zhi">Yan Zhi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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